Glycomics and proteomics platform

PAGès-P3M
Biology and health Platform Precision health Science for a changing planet

PAGés-P3M is the only French IBiSa labelled platform dedicated to the global analysis of single glycans, glycopeptides and whole glycoproteins. PAGés-P3M is part of the Structural and Functional Glucobiology Unit UMR8576 and benefits from 20 years of experience in the study of glycosylation. The platform has strong expertise in :
- NMR, gas chromatography and mass spectrometry for the identification of complex sugars and glycans,
- glycoproteomic analysis for the localization of glycosylations,
- mass spectrometry of whole glycoproteins for the quantification of glycoforms and the study of interactions with ligand or protein partners. We are open to all academic and industrial collaborations and propose services and training in line with our expertise.

Informations

Technical director: Nao Yamakawa
Scientific director: Jean-Michel Saliou (P3M)
Scientific director: Emmanuel Maes (PAGés)

https://ums-plbs.univ-lille.fr/les-plateformes-constitutives/glycomique-proteomique

Bâtiment C9, Avenue Mendeleiev
Campus Cité Scientifique, Université de Lille
59655 VILLENEUVE D'ASCQ

Mots-clés

Glycomics, Glycoproteomics, Proteomics, Polysaccharides, Oligosaccharides, Glycolipids, Gangliosides, Glycoproteins, Chromatography, Mass Spectrometry

Localisation

  • Composition and quantification of monosaccharide
  • Study of complex mixture of glycans, poly- and oligo-saccharides
  • Structural Glycobiology
  • NMR (nuclear magnetic resonance) spectroscopy
  • High throughput proteomics
  • Study of post-translational modifications
  • Glyco proteomics
  • Native mass spectrometry
  • Primary sequence, configuration and anomerism of glycans
  • Determination of the sialylation state
  • Biospecific molecular interaction study
  • Purification of biomolecules
  • Gas chromatography
  • Liquid chromatography
  • Mass Spectrometry
  • Multi-technique approaches
  • Quantification with or without labeling

Examples of projects:

  • Development of a method for the analysis of sialic acid type sugars (i.e. legionaminic acids, or pseudaminic acids) in bacteria 🡭
  • Systematic analysis of the glycome of the organs of a vertebrate 🡭
  • Development of a method to analyze the substitution rate of cyclodextrins
  • Co-development of a method to determine the specific N-glycoma of IgG glycosylation sites by analysis of glycopeptides in LC / MS2
  • Quantitative analysis of IgG glycopeptides
  • Proteomic analysis of protein maturation events PMID: 28958858
  • High-throughput proteomics on pull-down 🡭
  • Structural analysis of glycans and glycoconjugates in all their forms, ie glycolipids, glycoproteins, polysaccharides, oligosaccharides.
  • Analysis of monosaccharide composition (chemistry, gas phase, mass spectrometry, proton and heteroatom NMR)
  • Quantitative analysis of carbohydrates in complex mixtures and matrices
  • Quantitative proteomic analysis (immunopeptidome, interactome, complete proteome)
  • Glycoproteomic analysis
  • Native mass analysis (LC-MS, ESI-MS)
  • Quantification and dosage of monosaccharides
  • Sequence analysis (mass spectrometry, NMR)
  • Analysis of glycan maps of glycoproteins (mass spectrometry)
  • Purification (Liquid chromatography, low, medium and high pressure)
  • Implementation of protocols identified in the literature, optimization of protocols, development of techniques
  • Quantitative and qualitative analysis of glycans on their molecular support (lipid, protein, etc.)
  • Analysis of post-translational modifications
  • Thermal Proteom Profiling Analysis (CETSA)

The platform has a wide range of technologies to meet your requirements for the analysis of single glycans, glycopeptides and whole glycoproteins. We also offer the reservation of our machines to people who have previously followed a training with a member of the platform.

Polytech Montpellier, CHU-Nantes, Université du Littoral Côte d'Opale...
Nagoya University (Japan), University of Sheffield (UK)…

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