High throughput and high content screening platform

ARIADNE Criblage
Biology and health Platform Precision health

Our platform combines the know-how and equipment dedicated to small molecule and siRNA screening. Our equipment allows the complete or partial automation of the screening steps, from the plating of molecules from chemical or siRNA libraries, to the reading of results. We have access to chemical libraries and siRNA/miRNA libraries, and develop miniaturized (96, 384 well plates) and automated high throughput assays for in vitro, in bacterio, or in cellulo, target or phenotypic screening. The proposed tests include enzymatic assays, protein-protein interactions, cell-based assays, TSA, target engagement monitoring by CETSA and many others. Within the framework of a partnership agreement, projects can be pursued with Ariadne-Criblage experts towards target characterization and modulator optimization: target identification and engagement, medicinal chemistry, physicochemical properties measurement, ADME and pharmacokinetic studies in rodents.  We are open to all academic and industrial collaborations and propose training and services in line with our expertise.

Informations

Scientific director: Florence Leroux
Technical director: Alexandre Vandeputte

https://ums-plbs.univ-lille.fr/les-plateformes-constitutives/ariadne-criblage

Bâtiment Institut de Biologie de Lille et Bâtiment Debeyre, 1 Rue du Professeur Calmette
59000 LILLE

Mots-clés

High content and high throughput screening, Screening automation, Chemical library, siRNA / miRNA, Cell assays, Enzymatic assays, ADME, Pharmacokinetics, IBiSA

Localisation

  • High throughput testing 
  • Automated testing
  • High content and high throughput screening
  • Screening automation
  • Automated confocal microscopy
  • In vitro, in bacterio, or in cellulo screening, on targets or phenotypic 
  • Target characterization and modulator optimization
  • Development of miniaturized (96, 384 well plates) and automated high throughput tests for in vitro, in bacterio, or in cellulo, target or phenotypic screening 
  • High throughput and high content screening (HTS, HCS) in different containment environments (biosafety level 1 to 3 laboratories)
  • Enzymatic assays, phenotypic or target cell assays, protein-protein interactions in vitro or in cellulo, Thermal Shift Assay ...
  • Multimode reading (Fluorescence, fluorescence polarization, TR-FRET, BRET, Alpha ...)
  • Confocal microscopy and quantitative and automated image analysis

Our platform hosts various biology programs (microbiology, toxicology, oncology, neurology, etc.) and includes a wide range of equipment:

  • robotic assemblies for screening (BSL 2 and 3)
  • high throughput confocal microscopy
  • acoustic dispenser
  • liquid dispensers
  • robotic arms
  • fluorescence readers
  • automated incubators
  • PCR systems

Private
Bioversys, Apteeus, GSK

Academic
Institut Pasteur Paris; Institut Necker Paris; Paris Orsay; Université Paris Sud/ Institut Galien, Institut Polytechnique de Paris, Université Angers
Sciensano (Belgium), Paul Langerhans Institute Dresden (Germany)

Back