Laboratory of Virology

| ULR 3610
Biology and health Research unit Precision health

Our laboratory studies the mechanisms involved in the pathogenesis of enteroviral infections and in the development of type 1 diabetes. We are also looking for ways to control these infections. Enterovirus infections are studied through the coxsackievirus B4 (CVB4) serotype used as a model.

Informations

Director: Didier Hober

Centre Paul Boulanger, Boulevard du Professeur Jules Leclerc,
Hôpital Albert Calmettte, CHRU Lille
59037 LILLE

Mots-clés

Virus, Pathogenesis, Enterovirus, Antibodies, Type 1 diabetes, Virucide, Persistence, Viral inactivation, Prevention, Infections

Localisation

  • Conventional, molecular and serological virology
  • Enterovirus and in particular coxsackievirus B4
  • Viral parthogenesis of type 1 diabetes
  • In vitro and in vivo models (animals)
  • Detection and identification of enteroviruses
  • Culture in anaerobic environment (fermentations), gnotobiology
  • Pathogenesis of viral infection
  • Infection control and prevention. Viral inactivation (virucidal tests)
  • Viral infection markers
  • Detection and quantification of enteroviruses in biological media (isolation in cell culture)
  • Detection of enterovirus RNA in biological media (RT-PCR, semi-nested RT-PCR) and quantification of enterovirus RNA (quantitative real-time RT-PCR).
  • Detection of anti-enterovirus antibodies in biological media
  • Development of anti-enterovirus antibody detection tests
  • Detection of human and murine type 1 IFN by biological method.
  • Antiviral activity tests of molecules and products against enteroviruses
  • Virucidal activity tests of molecules and products against different families of viruses
  • Analysis of the intestinal flora (cultivable and non-cultivable bacteria)
  • Bacterial translocation in animals with controlled or conventional flora (NOD, DBA1, C3H)

We possess a wide range of equipment, including:

  • BSL2 and BSL2 + with PSM2 and CO2 incubators: cell and virus culture
  • Thermal cyclers and real-time PCR machine
  • Plate reader and washer (ELISA)
  • Delayed fluorescence reader (DELFIA)
  • UV microscope (fluorescence and immunofluorescence)
  • Electrophoresis, chromatography (gel filtration, affinity)
  • Ultracentrifuge
  • Anaerobic chamber, isolators, fermenters

Academic
Arts et Métiers ParisTech, INRIA
University of Exeter (UK), Tampere University (Finland), University of Liège (Belgium), University of Monastir (Tunisia), LIMMS (Japan), CHU Brazzaville (Congo)

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